GL-7ACA acylase
AssigneeFujisawa Pharmaceutical Co., Ltd.
InventorsIchiro Aramori, Masao Fukagawa, Hiroki Ono, Yosuke Ishitani, Mana Tsumura, Morita Iwami, Hitoshi Kojo
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Abstract
A GL-7ACA acylase having the following characteristics: (a) has ability to catalyze the enzymatic conversion of glutaryl 7-ACA, adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid, (b) has a molecular weight of 70,000 dalton (SDS-PAGE) and (c) has N-terminal amino acid sequence (SEQ ID NO: 1) thereof: Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-. A process for producing GL-7ACA acylase is also provided.
Claims4
An isolated and purified 7-(4carboxybutanamido)-3-acetoxymethyl-3-cephem-4-carboxylic acid (GL-7-ACA) acylase having the following characteristics:
- (a) catalyzes the enzymatic conversion of glutaryl 7 aminocephalosporic acid (7-ACA), adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid,
- (b) has a molecular weight of 70,000 daltons (SDS-PAGE)
- (c) has an N-terminal amino acid sequence (SEQ ID NO:
- 1) of the formula:
- Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu SEQ ID NO. 1
- and
- (d) is derived from Bacillus laterosporus J1.
Dependencies are inferred from the claim text.