Process for the detection of kidney tubule functioning
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsLothar Zimmerhackl, Rolf Kinne, Thomas Fabricius, Fricke Pietruschka, Mathias Brandis
Life of this patent
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Abstract
In the case of a process for the detection of the functionability of the kidney tubules, one specifically determines the content of THP in the urine as measure of the function of the kidney tubules. One uses this process for the control of the transplant rejection after a kidney transplantation, for the detection of blood supply disturbances of the kidney and of kidney damages produced by prior oxygen deficiency supply. Processes for determining kidney function by measuring Tamm-Horsfall protein (THP) using a specific monoclonal antibody are disclosed.
Claims9
A process for the detection of the level of functioning kidney tubules for a transplanted kidney function, a blood supply disturbance or kidney damage in a patient comprising
- determining the content of THP in a urine sample of said patient by immunoassay wherein a monoclonal antibody 2B3 (ECACC No. 88092301) reacts specifically with the protein component of urinary THP,
- by incubating the sample solution with the monoclonal antibody 2B3 and a labelled polyclonal specifically bindable with urinary THP or the complex of THP and the monoclonal antibody 2B3,
- and measuring the amount of the label as a measure of the amount of urinary THP to determine transplanted kidney function, extent of blood supply disturbance or extent of kidney damage.
A process for the detection of the level of functioning kidney tubules for transplanted kidney function, blood supply disturbance or kidney damage in a patient comprising
- determining the content of THP in a urine sample of said patient by immunoassay wherein a monoclonal antibody 2B3 (ECACC No. 88092301) reacts specifically with the protein component of urinary THP,
- by incubating the sample solution with the monoclonal antibody 2B3, wherein the 2B3 is bound to a solid phase or in an immobilizable form, and a labelled polyclonal antibody specifically bindable with urinary THP or the complex of THP and 2B3,
- separating the solid from the liquid phase and measuring the amount of label in one of the two phases as a measure of the amount of urinary THP to determine transplanted kidney function, extent of blood supply disturbance or extent of kidney damage.
Hybridoma cell line 2B3 (ECACC number 88092301).
A monoclonal antibody 2B3 as produced by the hybridoma cell line 2B3 (ECACC No. 88092301).
Dependencies are inferred from the claim text.