Back to results
US 5,310,651 AGranted
DNA probes of human immunodeficiency virus type 2 (HIV-2), and methods employing these probes for dectecting the presence of HIV-2
AssigneeInstitut Pasteur
InventorsMarc Alizon, Luc Montagnier, Denise Guetard, Francoise Brun-Vezinet, Francois Clavel
Life of this patent
Estimating the term…
Abstract
A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.
Claims4
An in vitro diagnostic method for the diagnosis of a human immunodeficiency virus type 2 (HIV-2) infection comprising the steps of:
- (a) contacting nucleic acid from a body sample suspected of containing viral genetic material with a detectable nucleic acid probe in a hybridization solution to form a mixture of nucleic acids, wherein said nucleic acid probe is selected from the group consisting of:
- i) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-626;
- ii) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-627;
- and
- iii) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-628;
- (b) washing the mixture of nucleic acids with a wash solution, and
- (c) detecting the formation of hybrids, wherein steps (a) and (b) are performed under conditions that allow generation of a strong hybridization signal in the presence of genomic nucleic acid of HIV-2 and a faint hybridization signal in the presence of genomic nucleic acid of HIV-1,
- wherein said detectable nucleic acid probe is such that (a) hybridization of the nucleic acid probe with nucleic acids of HIV-2 under hybridization conditions can be strongly detected, (b) hybridization of the nucleic acid probe with nucleic acids of STLV-III.sub.mac under hybridization conditions can be faintly detected, and (c) hybridization of the nucleic acid probe with nucleic acids of HIV-1 under hybridization conditions cannot be detected;
- and further wherein said hybridization conditions comprise contacting said probe with said HIV-2, STLV-III.sub.mac, or HIV-1 in a hybridization solution consisting essentially of about 5.times.SSC, about 5.times.Denhart, and about 50% formamide at about 42.degree. C. followed by washing with a wash solution consisting essentially of about 0.1.times.SSC and about 0.1% SDS at about 65.degree. C.
6 more elements
A process for detecting the presence of a human immunodeficiency virus type 2 (HIV-2) comprising:
- (a) providing a sample suspected of containing viral genetic material;
- (b) contacting said sample with a nucleic acid probe, wherein said nucleic acid probe is selected from the group consisting of:
- i) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-626;
- ii) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-627;
- and
- iii) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-628;
- and
- (c) detecting the formation of hybrids, wherein said nucleic acid probe is capable of producing a strong hybridization signal in the presence of genomic nucleic acid of HIV-2, a weak hybridization signal in the presence of genomic nucleic acid of SIV and faint or no hybridization signal in the presence of genomic nucleic acid of HIV-1, wherein said sample is contacted with said probe in a hybridization solution consisting essentially of about 5.times.SSC, about 5.times.Denhart, and about 50% formamide at about 42.degree. C. followed by washing with a wash solution consisting essentially of about 0.1.times.SSC and about 0.1% SDS at about 65.degree. C.
5 more elements
A nucleic acid probe capable of hybridizing under high stringency conditions to nucleic acid of a human immunodeficiency virus type 2 (HIV-2) to form a hybridized complex,
- wherein said hybridized complex is capable of being detected, and
- wherein said high stringency conditions comprise a hybridization condition and a wash condition that allow generation of a strong hybridization signal in the presence of genomic nucleic acid of HIV-2, a weak hybridization signal in the presence of genomic nucleic acid of SIV and a faint or no hybridization signal in the presence of genomic nucleic acid of HIV-1;
- and
- wherein said nucleic acid probe is selected from the group consisting of:
- i) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-626;
- ii) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-627;
- and
- iii) a probe consisting of HIV-2 nucleic acid deposited at the C.N.C.M. under Accession No. I-628;
- wherein said high stringency conditions comprise contacting said probe with said HIV-2, SIV, or HIV-1 in a hybridization solution consisting essentially of about 5.times.SSC, about 5.times.Denhart, and about 50% formamide at about 42.degree. C. followed by washing with a wash solution consisting essentially of about 0.1.times.SSC and about 0.1% SDS at about 65.degree. C.
6 more elements
Dependencies are inferred from the claim text.