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US 5,310,649 AGranted
Method for detecting species and biovars of Brucella
AssigneeThe Texas A&M University System
InventorsThomas A. Ficht, Blair A. Sowa, L. Garry Adams
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Abstract
A method for detecting Brucella infection in an animal which is reliable, rapid, and able to identify species and biovars of Brucella. The detection method includes the amplification of the omp2 gene locus of Brucella and analysis of restriction digestion fragments specific to Brucella and to individual species and groups of biovars of Brucella.
Claims19
A method for diagnosing Brucella infection comprising the steps of:
- releasing DNA from a test sample;
- releasing DNA from a test sample;
- amplifying at high stringency a gene sequence of a Brucella omp2 gene locus includes omp2a gene, omp2b gene, nucleic acid sequences intervening between the omp2a and omp2b genes, and approximately 300 nucleic acids flanking the omp2a and omp2b genes;
- and
- analyzing the amplified gene sequence to detect Brucella omp2 gene sequences diagnostic of Brucella.
2 more elements
A method for identifying a species or bovar of Brucella comprising the steps of:
- releasing DNA from a test sample;
- amplifying at high stringency a gene sequence of a Brucella omp2 gene locus from the released DNA, where the omp2 gene locus includes omp2a gene, omp2b gene, nucleic acid sequences intervening between the opm2a and omp2b genes, and approximately 300 nucleic acids flanking the omp2a and omp2b genes;
- and
- analyzing the amplified gene sequence to detect Brucella omp2 gene sequences diagnostic of a species or biovar of Brucella.
1 more element
Dependencies are inferred from the claim text.