On-column junction for capillary columns
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsRajeev Dadoo, Luis A. Colon, Harvey A. Fishman, Jason B. Shear, Richard N. Zare
Life of this patent
Estimating the term…
Abstract
An electrophoretic or chromatographic separation capillary containing a fluid defines a bore therein through which a sample travels and separates into components. The tube has a side wall defining a through hole therein which is surrounded by a medium including a substance. The substance is introduced into the capillary through the hole by means of gravity, pressure or electroosmosis. The substance introduced may be used to label sample components to enhance detection, or to enhance separation of sample components.
Claims29
An method for separating a sample into its components, employing a capillary tube defining a separation channel therein through which the sample travels and separates into its components, said tube having a side wall defining at least one hole therein, said hole extending through the side wall thereby permitting a fluid or particles to pass there through, said method comprising:
- surrounding said hole with a fluid medium containing a substance so that the medium is dispersed in a region larger than size of the hole;
- introducing a sample into the tube;
- causing at least a portion of said sample to travel through the tube towards the location of the hole;
- introducing said substance into the channel through the hole;
- and
- detecting the separated sample components at a location upstream or downstream from the hole.
An apparatus for use in separating a sample into its components, comprising:
- a capillary tube defining a separation channel therein through which the sample separates into its components, wherein one or more of said components travel in the tube defining an upstream and a downstream direction, said tube having a side wall defining at least one hole therein;
- a medium dispersed in a region larger than size of the hole and surrounding said hole, said hole extending through the side wall thereby permitting a fluid or particles to pass between said channel and said medium, said medium containing a substance;
- and
- a first device introducing said substance into the channel through the hole.
An apparatus for use in separating a sample into its components, comprising:
- a capillary tube defining a separation channel therein through which the sample separates into its components, wherein one or more of said components travel in the tube defining an upstream and a downstream direction, said tube having a side wall defining at least one hole therein;
- a first medium surrounding said hole, said hole extending through the side wall thereby permitting a fluid or particles to pass between said channel and said first medium, said first medium containing a substance;
- a second gel medium in the tube;
- and
- a first device introducing said substance into the channel through the hole.
Dependencies are inferred from the claim text.