Quantitation of samples utilizing capillary electrophoresis

A method of quantifying peptide-containing constituent species in a clinical sample by capillary zone electrophoresis comprising the following steps:

• a) adding at least one ionic species having a charge density of between about 0.02 and about 0.001 to the sample to form a clinical mixture, the charge density of the ionic species being greater than substantially all of the constituent species in the clinical sample;
• b) separating said clinical mixture into its constituent parts by capillary zone electrophoresis;
• c) detecting the ionic species and the peptide-containing constituent species in said mixture;
• and
• d) normalizing at least one peptide-containing constituent species by dividing the integrated area value of said peptide-containing species by the total integrated area values from all of the peptide-containing constituent species to obtain a first value;
• dividing the first value by a second value to obtain a third value, the second value being obtained by dividing the integrated area value of the ionic species by the total integrated area amounts from the constituent species;
• multiplying the third value by a fourth value, the fourth value being the dilution factor of the sample to obtain a fifth value;
• multiplying the fifth value by a sixth value, the sixth value being obtained by dividing the absorbance value of a fixed amount of the sample measured at a fixed wavelength by the absorbance value of about the same fixed amount of the ionic species measured at about the same fixed wavelength;
• and multiplying the sixth value by the concentration of the ionic species in the sample.

A method of quantifying peptide-containing constituent species in a sample by capillary electrophoresis comprising the following steps:

• a) adding at least one ionic species to the sample to form a mixture, the ionic species having a charge density less than substantially all of the peptide-containing constituent species in the sample,
• b) subjecting the mixture to capillary zone electrophoresis,
• c) detecting the ionic species and the peptide-containing constituent species in said mixture, and
• d) normalizing at least one peptide-containing constituent species relative to the detected ionic species,
• where the normalized value of step (d) is the quantified amount of said peptide-containing constituent species in the sample.

A method of quantifying peptide-containing constituent species in a clinical sample by capillary zone electrophoresis comprising the following steps:

• a) adding at least one ionic species having a charge density of between about 0.02 and about 0.001 to the clinical sample to form a clinical mixture, the charge density of the ionic species being less than substantially all of the peptide-containing constituent species in the clinical sample;
• b) separating said clinical mixture into its constituent parts by capillary zone electrophoresis;
• c) detecting the ionic species and the constituent peptide-containing species in said mixture;
• and
• d) normalizing at least one peptide-containing constituent species by dividing the integrated area value of said peptide-containing species by the total integrated area values from all of the constituent peptide-containing species to obtain a first value;
• dividing the first value by a second value to obtain a third value, the second value being obtained by dividing the integrated area value of the ionic species by the total integrated area amounts from the constituent peptide-containing species;
• multiplying the third value by a fourth value, the fourth value being the dilution factor of the clinical sample to obtain a fifth value;
• multiplying the fifth value by a sixth value, the sixth value being obtained by dividing the absorbance value of a fixed amount of the clinical sample measured at a fixed wavelength by the absorbance value of about the same fixed amount of the ionic species measured at about the same fixed wavelength;
• and multiplying the sixth value by the concentration of the ionic species in the clinical sample.