Method for classifying and counting leukocytes
AssigneeToa Medical Electronics Co., Ltd.
InventorsTakashi Sakata, Mitsue Ito
Life of this patent
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Abstract
A hematological specimen for classifying and counting leukocytes with a flow cytometer is prepared. A sample to be assayed is prepared by eliminating influences of erythrocytes from a hematological sample without changing leukocytes morphologically; adjusting the pH value to a level suitable for staining; and staining the leukocytes with at least two dyes including Astrazon Yellow 3G capable of specifically staining at least basophils and immature granulocytes and Neutral Red capable of specifically staining at least eosinophils. Thus leukocytes contained in the hematological sample can be classified at least three or six groups including immature granulocytes by measuring a single specimen with a flow cytometer.
Claims7
A method for classifying and counting leukocytes into at least three groups including immature granulocytes by assaying a single specimen with a flow cytometer, which comprises the following steps:
- (1) a step for adjusting a pH value of a hematological sample to a level suitable for staining;
- (2) a step for staining leukocytes contained in said hematological sample with a mixture of at least two dyes specified as follows:
- i) Astrazon Yellow 3G capable of staining at least basophils and immature granulocytes;
- and
- ii) Neutral Red capable of staining at least eosinophils;
- and
- (3) a step for counting and classifying the leucocyte with a flow cytometer.
A method for classifying and counting leukocytes into at least six groups including immature granulocytes by assaying a single specimen with a flow cytometer, which comprises the following steps:
- (1) a step for eliminating influences of erythrocytes from a hematological sample without changing the leukocytes morphologically;
- (2) a step for adjusting a pH value of said hematological sample to a level suitable for staining;
- (3) a step for staining leukocytes contained in said hematological sample with a mixture of at least two dyes specified as follows:
- i) Astrazon Yellow 3G capable of staining at least basophils and immature granulocytes;
- and
- ii) Neutral Red capable of staining at least eosinophils;
- and
- (4) a step for counting and classifying leucocyte with a flow cytometer.
Dependencies are inferred from the claim text.