Electrogenerated luminescence in solution
OwnerJouko J. Kankare, Haapakka, Keijo E.inventors
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Abstract
A method whereby the presence and/or amount of a chemical moiety containing terbium is determined by applying an electrical pulse to an electrode immersed in a solution and measuring the delayed light emission after some time from the end of the pulse, said chemical moiety being either bound to said electrode and/or present in said solution and said emitted light being taken as an indication of the amount of the chemical moiety present in the proximity of said electrode.
Claims4
A method of determining the amount of a chemical moiety containing terbium, said chemical moiety being in an aqueous solution, said chemical moiety having the formula Tb-Z-wherein:
- Z has the formula:
- ##STR6## consisting essentially of the steps of:
- (a) contacting a sample containing said chemical moiety with two electrodes one of which is made of aluminum;
- (b) applying an electrical pulse across said electrodes;
- (c) measuring the emitted light originating from one of the said electrodes starting after a delay of 0.1 to 0.5 ms from the end of the said electrical pulse and extending the measurement up to ten milliseconds;
- (d) measuring the amount of said chemical moiety on the basis of the light intensity emitted in step (c).
A method of determining the amount of the antigen human pancreatic phospholipase A.sub.2 in a sample which contains a chemical moiety containing terbium, consisting essentially of the steps of:
- (a) contacting a sample containing said chemical moiety with two electrodes one of which is made of aluminum;
- (b) applying an electrical pulse across said electrodes;
- (c) measuring light originating from one of the said electrodes starting after a delay of 0.1 to 0.5 ms from the end of the said electrical pulse and extending the measurement up to ten milliseconds;
- (d) measuring the amount of said chemical moiety on the basis of the light intensity emitted in step (c), said chemical moiety having the formula:
- Y.sub.P -L.sub.m -(Q-Z-Tb).sub.n
- wherein:
- Z has the formula:
- ##STR7## Y is an antibody of human pancreatic phospholipase A.sub.2 ;
- L is a bivalent linking group selected from the group consisting of ureido, thioureido, amide, substituted amide, thioether, sulfonamide, and a N-substituted sulfonamide;
- Q is a spacer arm consisting of a molecular chain which separates the group Z and said antibody of human pancreatic phospholipase A.sub.2 and is selected from the group consisting of --C.sub.6 H.sub.4 --CO, --C.sub.6 H.sub.4 --CH.sub.2 --, --C.sub.6 H.sub.4 --CH.sub.2 CH.sub.2 -- and --(CH.sub.2).sub.r, wherein --C.sub.6 H.sub.4 -- stands for 1,2-, 1,3- or 1,4-phenylene and r is an integer from 1 to 6;
- said antibody of human phospholipase A2 being attached to Z through one or more linking groups L and spacer arm Q;
- n is an integer equal to or greater than 1;
- p is an integer greater than zero;
- m is an integer equal to or greater than p.
Dependencies are inferred from the claim text.