Biocompatibility of solid surfaces
AssigneeMedtronic, Inc.
InventorsPatrick T. Cahalan, Michel Verhoeven, Marc Hendriks, Linda Cahalan
Life of this patent
Estimating the term…
Abstract
An improved spacer material for improving the biocompatibility of a biomaterial and a method for making it in which a polyalkylimine is covalently attached to an aminated substrate and combined with a crosslinking agent which is at least difunctional in aldehyde groups. The polyalkylizine can be, for example, polyethyleneimine and the crosslinking agent can be, for example, glutaraldehyde. Preferably, the crosslinking agent is applied in dilute solution and at a pH suitable to accomplish light crosslinking of the polyalkylimine and also provide aldehyde linkages at the interface between the biomolecule and the spacer.
Claims19
A method for making a spacer for attaching a biomolecule to a solid surface comprising the steps of:
- (a) aminating the solid surface by grafting acrylamide onto the solid surface;
- (b) covalently bonding a polyalkylimine to the aminated surface;
- and
- (c) applying a crosslinking agent which is at least difunctional in aldehyde groups to the covalently bonded polyalkylimine.
A method for covalent coupling of a polyalkylimine to a solid surface comprising the steps of:
- (a) aminating the solid surface by grafting acrylamide onto the solid surface;
- (b) attaching to the aminated surface a spacer molecule having at least one primary amine group extended away from the surface;
- (d) contacting the activated spacer with a polyalkylimine.
A method for attaching a cellular adhesive molecule to a solid surface comprising the steps of:
- (a) aminating the solid surface by grafting acrylamide onto the solid surface;
- (b) covalently bonding to the aminated solid surface a polyalkylimine;
- (c) applying a crosslinking agent to the covalently bonded polyalkylimine, said crosslinking agent being at least difunctional in aldehyde groups;
- (d) coupling a cellular adhesive molecule to the treated polyalkylimine.
Dependencies are inferred from the claim text.