Method to directly radiolabel antibodies for diagnostic imaging and therapy
AssigneeThomas Jefferson University
InventorMathew L. Thakur
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Abstract
The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.
Claims8
A kit suitable for directly labeling a protein with radionuclides for use in diagnostic imaging and therapy comprising a quantity of protein and a quantity of sodium ascorbate sufficient to reduce a controlled number of disolfide groups in the protein to sulfhydryls;
- and a quantity of sodium dithionite or stannous chloride sufficient to reduce the labeling radionuclide.
Dependencies are inferred from the claim text.