DNA encoding glucanase enzymes
OwnerAizo Matsushiroinventor
Life of this patent
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Abstract
Disclosed is the isolation and cloning of genes encoding glucanohydrolase enzymes and the expression and secretion of exogenous glucanase gene products in host cells.
Claims2
A recombinant DNA molecule consisting of segments of DNA from different genomes which have been joined end-to-end outside of living cells and which have the capacity to transform a host and to be maintained therein, and the progeny thereof, comprising a DNA sequence selected from the group consisting of:
- (a) a DNA fragment present in Arthrobacter sp. CB-8 (FERM BP-995) and encoding .alpha.-1,6-glucan 6-glucanohydrolase,
- (b) DNA sequences which hybridize to the foregoing DNA under strigent conditions and which code on expression for .alpha.-1,6-glucan 6-glucanohydrolase, and
- (c) DNA sequences which code on expression for an .alpha.-1,6-glucan 6-glucanohydrolase enzyme of the type coded on expression by any of the forgoing DNA sequences and fragments,
- said DNA sequences and fragments being operatively linked to an expression control sequence in said recombinant DNA molecule.
A recombinant DNA molecule consisting of segments of DNA from different genomes which have been joined end-to-end outside of living cells and which have the capacity to transform a host and to be maintained therein, and the progeny thereof, comprising a DNA molecule selected from the group consisting of
- (a) a DNA fragment present in Bacillus circulans BC-8 (FERM BP-733) and encoding exclusively .alpha.-1,3glucan 3-glucanohydrolase,
- (b) DNA molecules which hybridize to the foregoing DNA under strigent conditions and which code on expression for exclusively .alpha.-1,3-glucan 3-glucanohydrolase, and
- (c) DNA molecules which code on expression for an .alpha.-1,3-glucan 3-glucanohydrolase enzyme of the type coded for on expression by any of the foregoing DNA molecules or fragments,
- said DNA molecules and fragments thereof being operatively linked to a promoter, a ribosome-binding site, and a signal in said recombinant DNA molecule.
Dependencies are inferred from the claim text.