Activated carbamates compounds
AssigneeMillipore Corporation
InventorsSteven A. Cohen, Dennis P. Michaud
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Abstract
A class of novel heterocyclic aromatic carbamate compounds is described. A method of derivatizing amine-functional compounds with the novel carbamates is also disclosed. The derivatives can be detected using a fluorescence detector. The present composition and method allow femtomole quantities of amine compounds to be detected and measured.
Claims4
A heterocyclic activated carbamate compound having the formula:
- ##STR7## wherein Ar is quinolyl, acridinyl, coumarin or fluorescein which may be substituted with alkyl, alkoxy, OH, halo, amino, sulfur, oxygen or phenyl.
A heterocyclic aromatic carbamate compound selected from the group consisting of:
- a) 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate,
- b) 3-aminoquinolyl-N-hydroxysuccinimidyl carbamate,
- c) 5-aminoquinolyl-N-hydroxysuccinimidyl carbamate,
- d) 5-aminoisoquinolyl-N-hydroxysuccinimidyl carbamate,
- e) 6-amino-4-methylquinolyl-N-hydroxysuccinimidyl carbamate,
- f) 6-amino-2,4-dimethylquinolyl-N-hydroxysuccinimidylcarbamate,
- g) 6-amino-2-phenylquinolyl-N-hydroxysuccinimidyl-carbamate,
- h) 6-amino-2-methoxy-4-methylquinolyl-N-hydroxysuccinimidylcarbamate, and
- i) 4-aminoquinaldine-N-hydroxysuccinimidyl carbamate.
Aminoquinolyl-N-hydroxysuccinimidyl carbamate.
A heterocyclic aromatic carbamate compound selected from the group consisting of:
- a) 9-aminoacridine-N-hydroxysuccinimidyl carbamate,
- b) 2-aminoacridine-N-hydroxysuccinimidylcarbamate,
- c) luminol-N-hydroxysuccinimidylcarbamate,
- d) isoluminol-N-hydroxysuccinimidylcarbamate,
- e) 7-amino-4-methylcoumarin-N-hydroxysuccinimidylcarbamate,
- f) 7-amino-4-(trifluoromethyl)coumarin-N-hydroxysuccinimidylcarbamate,
- g) 4'-(aminomethyl)fluorescein-N-hydroxysuccinimidylcarbamate,
- h) 5-(aminomethyl)fluorescein-N-hydroxysuccinimidylcarbamate,
- i) 5-aminoeosin-N-hydroxysuccinimidylcarbamate;
- and
- j) Cascade Blue ethylenediamine-N-hydroxysuccinimidylcarbamate.
Dependencies are inferred from the claim text.