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US 5,296,592 AGranted
Process for purifying interleukin-1 receptors
AssigneeImmunex Corporation
InventorsSteven K. Dower, Carl J. March, John E. Sims, David L. Urdal
Life of this patent
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Abstract
Mammalian Interleukin-1 receptor proteins (IL-1Rs), DNAs and expression vectors encoding mammalian IL-1Rs, and processes for producing mammalian IL-1Rs as products of cell culture, including recombinant systems, are disclosed.
Claims6
A process for purifying a biologically active mammalian type I IL-1 receptor, which has a full length native form having a molecular weight of about 82 kd, comprising:
- (a) applying a sample comprising mammalian IL-1 receptor to an affinity matrix comprising a ligand selected from the group consisting of an antibody specific to the type I IL-1 receptor or an IL-1 molecule bound to an insoluble support;
- and
- (b) eluting the IL-1 receptor from the affinity matrix.
Dependencies are inferred from the claim text.