Method for controlling o-desulfation of heparin and compositions produced thereby
AssigneeGlycomed Incorporated
InventorsKevin R. Holme, Patrick N. Shaklee, David J. Tyrrell
Life of this patent
Estimating the term…
Abstract
Methods of making and using, as prophylatics or therapeutics for preventing or treating certain diseases including cancer, O-desulfated heparin compositions, preferably 2-O, 3-O desulfated heparin compositions, wherein the methods permit regulating the degree of 2-O, 3-O desulfation to produce compositions that are variably desulfated including about 99% and 75% desulfated at the 2-O and 3-O positions, respectively.
Claims8
A composition comprising substantially unfragmented 2-O 3-O desulfated mucosal heparin, or 2-O, 3-O desulfated mucosal heparin fragments, wherein said substantially unfragmented 2-O, 3-O desulfated heparin, or 2-O, 3-O desulfated heparin fragments are at least about 96.7% to about 99% and 75% desulfated at the 2-O and 3-O positions, respectfully, wherein said fragments have an average molecular weight range of about 2-6.5 kd.
A pharmaceutical composition comprising substantially unfragmented 2-O, 3-O desulfated mucosal heparin or 2-O, 3-O desulfated mucosal heparin fragments and a pharmaceutically acceptable excipient wherein said unfragmented 2-O, 3-O desulfated heparin, or 2-O, 3-O desulfated heparin fragments are at least about 96.7% to about 99% and 75% desulfated at the 2-O and 3-O positions, respectfully, wherein said fragments have an average molecular weight range of about 2-6.5 kd.
A method for treating disease in an animal host comprising administering to said animal host an effective amount of a composition comprising substantially unfragmented 2-O, 3-O desulfated mucosal heparin or 2-O, 3-O desulfated mucosal heparin fragments wherein said composition are at least about 96.7% to about 99% and 75% desulfated at the 2-O and 3-O positions, respectively, wherein said fragments have an average molecular weight range of about 2-6.5 kd.
A method for inhibiting angiogenesis in an animal suffering from unwanted angiogenesis, comprising administering to said animal an effective amount of a 2-O, 3-O desulfated mucosal heparin or heparin fragment composition wherein said 2-O, 3-O desulfated mucosal heparin or heparin fragment composition are at least about 96.7% to about 99% and 75% desulfated at the 2-O and 3-O positions, respectively, wherein said fragments have an average molecular weight range of about 2-6.5 kd.
Dependencies are inferred from the claim text.