Method and compositions using polypeptides of arabidopsis thaliana
AssigneeBoard of Trustees operating Michigan State University
InventorMichael F. Thomashow
Life of this patent
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Abstract
A method for treating chemical or biological materials with a polypeptide derived from a RNA encoded by a cDNA of Arabidopsis thaliana to prevent freezing or heat damage is described In particular, a novel cDNA and polypeptide, COR15, encoded thereby is described.
Claims7
A method for protecting a biomembrane or protein material in vitro from freezing damage which comprises applying a polypeptide selected from the group consisting of polypeptide-1, polypeptide-2, polypeptide-3, polypeptide-4, polypeptide 5 and mixtures thereof which polypeptide is derived from Arabidopsis thaliana, which polypeptide is 6.6, 15, 24, 46 and 160 kilodaltons in mass as measured by SDS-PAGE, respectively, to the material in vitro wherein the polypeptide protects the material from freezing damage.
A method for protecting a biomembrane or protein material in vitro from freezing damage which comprises admixing a cryoprotective amount of a polypeptide selected from the group consisting of polypeptide-1, polypeptide-2, polypeptide-3, polypeptide-4, polypeptide 5 and mixtures thereof which polypeptide is derived from Arabidopsis thaliana, which polypeptide is 6.6, 15, 24, 47 and 160 kilodaltons in mass as measured by SDS-PAGE, respectively, with the material in vitro, wherein the polypeptide protects the material from freezing damage.
A composition which comprises in admixture:
- (a) a biomembrane or protein material which is to be protected in vitro against freezing or water stress damage;
- and
- (b) a polypeptide substantially free of Arabidopsis thaliana proteins and selected from the group consisting of polypeptide 1, polypeptide-2, polypeptide-3, polypeptide 4 and polypeptide-5 and mixtures thereof which polypeptide is derived from Arabidopsis thaliana, which polypeptide is 6.6, 15, 24, 47 and 160 kilodaltons in mass as measured by SDS-PAGE, respectively, with the material in vitro in an amount sufficient for protection between about 10.sup.-3 and 10.sup.5 part per part of the material.
Dependencies are inferred from the claim text.