US 5,296,366 A · Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant DNA and transformed microorgnaisms

Method for the isolation and expression of a gene which codes for streptokinase, nucleotide sequence obtained, recombinant DNA and transformed microorgnaisms

AssigneeCentro de Ingenieria Genetica y Biotecnologia

InventorsMario P. E. Garcia, Aimee P. Felipe, Roger R. Chaplen, Ricardo S. Doce, Luciano F. H. Marrero, Pedro R. Collazo, Anaisel C. Ramirez, Emilio A. M. Munoz, Walfrido B. Martinez, Magalys C. Somavilla, Alicia P. Fernandez, Jose d. d. Garcia, Luis S. H. Martinez

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Abstract

The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms. The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which it includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase. The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.

Claims26

Dependent claims

A method for producing streptokinase by expression of a gene encoding said streptokinase which comprises the steps of transforming a host cell with an expression vector containing a streptokinase gene (skc-2) consisting essentially of the nucleotide sequence SEQ ID NO:

1 in the Sequence Listing operably linked to an effective promoter and transcription terminator, growing said transformed host cell and isolating the streptokinase so produced.

Dependencies are inferred from the claim text.