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US 5,294,550 AGranted
Method of culturing Mishima-saiko
AssigneeShiseido Company, Ltd.
InventorsKen Kusakari, Mineyuki Yokoyama, Mitsuo Yanagi
Life of this patent
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Abstract
An improved process for the organ cultivation of a Mishima-saiko root is provided. In the cultivation of a Mishima-saiko root, by carrying out the cultivation while regulating the saccharide concentration to be less than 2%, until the adventitious roots have spread out, and optionally adding an additional saccharide, the productivity of saiko-saponin can be enhanced.
Claims5
A process for producing saponin comprising the steps of:
- a) culturing Mishima-saiko root as an explant in a medium having a carbohydrate concentration less than 2% by weight of the total medium for a time sufficient to effect a maximum number of lateral roots and produce saponin;
- b) further culturing the Mishima-saiko root in a medium regulated at a carbohydrate concentration of at least 2% by weight of the total medium to produce saponin;
- and
- c) recovering the saponin.
1 more element
Dependencies are inferred from the claim text.