Biologically active factor
AssigneeResearch Corporation Technologies, Inc.
InventorsPatrick S. Gentile, Charlie R. Mantel, Hal E. Broxmeyer
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Abstract
The invention comprises a factor having the following characteristics: a) It inhibits granulocyte-macrophage colony and cluster formation; b) It has a molecular weight of about 8 kDa as determined by SDS-PAGE; c) It has a weak anionic charge at pH 7.4 as shown by anion exchange chromatography; d) It has a flattened isoelectric titration curve as shown by anion exchange chromatography; and d) It is a protein. The invention also comprises methods of making and using the factor and compositions comprising the factor.
Claims6
A method of making a biologically active protein factor comprising:
- injecting a mouse with iron-saturated, endotoxin-depleted lactoferrin or with acidic isoferritin;
- and
- conditioning a culture medium from a sufficient time with bone marrow or spleen cells removed from the mouse a sufficient time after the injection of the mouse with lactoferrin or acidic isoferritin to effect production of the factor;
- and
- purifying the factor which:
- a) inhibits granulocyte-macrophage colony and cluster formation;
- b) has a molecular weight of about 8 kDa as determined by SDS-PAGE;
- c) has a weak anionic charge at pH 7.4 as shown by anion exchange chromatography;
- and
- d) has a flattened isoelectric titration curve as shown by anion exchange chromatography.
A method of making a conditioned medium containing a biologically active protein factor, the method comprising:
- injecting a mouse with iron-saturated, endotoxin-depleted lactoferrin or with acidic isoferritin;
- and
- conditioning a culture medium for a sufficient time with bone marrow or spleen cells removed from the mouse a sufficient time after the injection of the mouse with lactoferrin or acidic isoferritin to effect production of the factor which
- a) inhibits granulocyte-macrophage colony and cluster formation;
- b) has a molecular weight of about 8 kDa as determined by SDS-PAGE;
- c) has a weak anionic charge at pH 7.4 as shown by anion exchange chromatography;
- and
- d) has a flattened isoelectric titration curve as shown by anion exchange chromatography.
Dependencies are inferred from the claim text.