Ethanol analytical element
AssigneeEastman Kodak Company
InventorsDaniel S. Daniel, Karen L. Warren, James R. Schaeffer
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Abstract
A multilayer analytical element for quantitatively assaying ethanol comprising a tetrazolium salt, alcohol dehydrogenase, NAD.sup.+, and an electron transfer agent characterized in that the layer comprising the electron transfer agent also includes a polymer having recurring negatively charged groups and the NAD.sup.+ is in a different layer is disclosed.
Claims8
A multilayer analytical element for quantitatively assaying ethanol comprising a tetrazolium salt, alcohol dehydrogenase, NAD.sup.+, and an electron transfer agent wherein the layer comprising the electron transfer agent also includes a polymer having recurring negatively charged groups, and the alcohol dehydrogenase is in a different layer.
A multilayer element for the quantitative assay of ethanol in aqueous fluids, comprising a combination reagent/spreading layer and a reagent layer comprising a first and second zone, wherein the reagent/spreading layer comprises from 1 to 8 g/m.sup.2 of a tetrazolium salt and from 2 to 8 g/m.sup.2 of NAD.sup.+ ;
- the first reagent zone comprises from 5,000 to 20,000 U/m.sup.2 of alcohol dehydrogenase;
- and the second reagent zone comprises from 0.01 to 0.2 g/m.sup.2 of phenothiazine methosulfate and from 0.1 to 4 g/m.sup.2 poly(methyl acrylate-co-2-sulfo-1,1-di-methylethyl acrylamide-co-2-acetoacetoxyethyl methacrylate).
Dependencies are inferred from the claim text.