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US 5,294,538 AGranted
Method of screening for antimitotic compounds using the CDC25 tyrosine phosphatase
AssigneeCold Spring Harbor Labs.
InventorDavid H. Beach
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Abstract
A method of identifying compounds or molecules which alter (enhance or inhibit) stimulation of kinase activity of pre-MPF and, thus, alter (enhance or inhibit) activation of MPF and entry into mitosis. The present method thus makes it possible to identify compounds or molecules which can be administered to regulate the cell cycle; such compounds are also the subject of this invention.
Claims4
A method of identifying a compound which is an inhibitor of human CDC25 tyrosine phosphatase activity, comprising the steps of:
- a) combining:
- 1) a compound to be assessed;
- 2) CDC25 from a human source;
- and
- 3) p-nitrophenyl phosphate;
- b) maintaining the combination produced in (a) under conditions appropriate for CDC25 to act upon p-nitrophenyl phosphate;
- and
- c) determining the extent of action of said CDC25 upon p-nitrophenyl phosphate relative to a control, said control comprising human CDC25 and p-nitrophenyl phosphate in the absence of said compound to be assessed;
- wherein if human CDC25 present in the combination produced in acts upon p-nitrophenyl phosphate to an extend less by at least 5-10% than human CDC25 present in the control, the compound is an inhibitor of human CDC25 tyrosine phosphatase activity.
6 more elements
Dependencies are inferred from the claim text.