Conjugates
AssigneePB Diagnostic Systems, Inc.
InventorPaul S. Palumbo
Life of this patent
Estimating the term…
Abstract
A compound represented by the formula ##STR1## wherein X is a spacer group. The compound is useful for conjugating a compound having an alcohol group or an amine group to a compound having a thiol group. The compound can be used to conjugate a biologically active group such as an antigen to a protein such as an enzyme to provide an enzyme-labeled antigen for use in enzyme-amplified immunoassay methods for analytes or metabolites in sample fluids. The compound can also be used to immobilize a material such as a protein to a solid support.
Claims14
A conjugate represented by the formula ##STR7## wherein X is alkylene, an aromatic carbocyclic moiety or a saturated carbocyclic moiety.
- --R is the residue of an antigen having an alcohol and/or an amine group, where R is linked to the carbonyl group through an oxygen atom or a nitrogen atom;
- and
- --R.sub.1 is the residue of a protein having a thiol group, where R.sub.1 is linked to the maleiimide moiety through a sulfur atom.
A conjugate represented by the formula ##STR8## where X is phenyl, naphthyl or phenyl or naphthyl substituted with one or two alkylene groups having from 1 to 6 carbon atoms, attachment to the --NH moiety and/or the maleiimide moiety being effected through one of said alkylene groups;
- --R is the residue of an antigen having an alcohol and/or an amine group, where R is linked to the carbonyl group through an oxygen atom or a nitrogen atom;
- and
- --R.sub.1 is the residue of a protein having a thiol group, where R.sub.1 is linked to the maleiimide moiety through a sulfur atom.
A conjugate represented by the formula ##STR9## wherein X is a saturated carbocyclic moiety substituted with one or two alkylene groups having from 1 to 6 carbon atoms, attachment to the --NH moiety and/or the maleiimide moiety being effected through one of said alkylene groups;
- --R is the residue of an antigen having an alcohol and/or an amine group, where R is linked to the carbonyl group through an oxygen atom or a nitrogen atom;
- and
- --R.sub.1 is the residue of a protein having a thiol group, where R.sub.1 is linked to the maleiimide moiety through a sulfur atom.
A solid support for use in immunoassays comprising a conjugate represented by the formula ##STR10## where X is alkylene, an aromatic carbocyclic moiety or a saturated carbocyclic moiety;
- --R is the residue of a protein having an alcohol and/or an amine group, where R is linked to the carbonyl group through an oxygen atom or a nitrogen atom;
- and
- R.sub.1 is the residue of a solid support material having a thiol group, where R.sub.1 is linked to the maleiimide moiety through a sulfur atom.
A solid support for use in immunoassays comprising a conjugate represented by the formula ##STR11## wherein X is alkylene, an aromatic carbocyclic moiety or a saturated carbocyclic moiety;
- --R is the residue of a solid support material having an alcohol and/or an amine group, where R is linked to the carbonyl group through an oxygen atom or a nitrogen atom;
- and
- R.sub.1 is the residue of a protein having a thiol group, where R.sub.1 is linked to the maleiimide moiety through a sulfur atom.
Dependencies are inferred from the claim text.