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US 5,294,403 AGranted
Kit for the determination of bilirubin in urine
AssigneeDiesse Diagnostica Senese S.r.l.
InventorFranco Meiattini
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Abstract
A kit including a reagent containing a buffer suitable for maintaining the pH of the reagent between 3-7, sulfanilic acid or sulfanilamide, a cupric soluble salt and an alkaline metal nitrite, for the detection and determination of bilirubin in the urine providing precise results and ready automation of the procedure, characterized in that it is a stable combination of all the substances used for such analysis.
Claims9
A reagent kit for determining the concentration of bilirubin in urine, consisting essentially of, in two separate containers:
- (A) a first aqueous reagent consisting essentially of 10-5,000 mmol/l of a buffer suitable to maintain the pH of said reagent between 3-7 and inert to cupric ions, 5-200 mmol/l of an aromatic compound selected from the group consisting of sulfanilic acid and sulfanilamide, 1-30 mmol/l of a soluble cupric salt, and 0-10% w/v of an anionic or non-ionic surfactant;
- and
- (B) a second aqueous reagent consisting essentially of 10-1,000 mmol/l of an alkaline metal nitrite and 0-100% w/v dimethylsulfoxide.
Dependencies are inferred from the claim text.