Method for separation of .beta.-amylase
AssigneeDirector of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
InventorChikafusa Fukazawa
Life of this patent
Estimating the term…
Abstract
Disclosed is a method of separating .beta.-amylase from a solution containing .beta.-amylase, in which the solution is treated with an .alpha.-cyclodextrin fixed water-insoluble high polymer compound in the presence of ammonium sulfate to form an adsorbed composite of the high polymer compound and .beta.-amylase and soluble impurities not adsorbed to the high polymer compound are separated and removed. By the method, a high-purity .beta.-amylase, which is highly useful in the starch saccharification industry, may specifically and efficiently be isolated from a .beta.-amylase-containing mixture solution.
Claims3
A method of separating .beta.-amylase from a solution containing .beta.-amylase, which comprises treating said solution with an .alpha.-cyclodextrin fixed water-insoluble high polymer compound in the presence of ammonium sulfate to form an adsorbed composite of the high polymer compound and .beta.-amylase and separating soluble impurities not adsorbed to the high polymer compound to remove the impurities.
Dependencies are inferred from the claim text.