Method for producing a carrier for cation exchange liquid chromatography and a method for determining glycosylated hemoglobins using the carrier

A production method of a carrier for cation exchange liquid chromatography comprising the steps of:

• providing an aqueous dispersion in which hydrophobic crosslinked polymer particles to which a polymerization initiator is adhered are dispersed;
• and
• adding a polymerizable monomer having a carboxyl group to the aqueous dispersion and polymerizing the monomer on the surface of the hydrophobic crosslinked polymer particle, thereby forming a polymer layer having carboxyl groups on the surface of the hydrophobic crosslinked polymer particle to obtain a coated polymer particle,
• wherein said hydrophobic crosslinked polymer is obtained by homopolymerizing or copolymerizing at least one kind of a hydrophobic crosslinkable monomer, or copolymerizing at least one kind of a hydrophobic crosslinkable monomer and at least one kind of a hydrophobic noncrosslinkable monomer, where said hydrophobic crosslinkable monomer is selected from the group consisting of poly(meth)acrylates and aromatic compounds having at least two vinyl groups;
• and further wherein said polymerizable monomer having a carboxyl group selected from the group consisting of acrylic acid, methacrylic acid, maleic acid, fumaric acid and crotonic acid.