Catalyst for preparing polyacrylamide gel which improves the detection of biomaterials by silver staining
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsDenis F. Hochstrasser, Carl R. Merril
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Abstract
A polyacrylamide gel comprising acrylamide and diacrylylpiperazine. A method for providing a polyacrylamide gel comprising employing a catalyst system which comprises dimethylpiperazine, sodium thiosulfate or a mixture thereof and ammonium or potassium persulfate. The use of the gel as the matrix in a silver staining procedure for the detection of biomaterials, provides for the obtainment of reduced background staining.
Claims15
A method for preparing a polyacrylamide gel comprising polymerizing acrylamide with diacrylylpiperazine in the presence of a catalyst system which comprises dimethylpiperazine in combination with ammonium persulfate or potassium persulfate.
An improved silver stain method utilizing a polyacrylamide gel for the detection of biomaterials with reduced background staining, wherein the improvement comprises the use of a polyacrylamide gel comprising a matrix of arylamide with diacrylylpiperazine polymerized in the presence of a catalyst system which comprises dimethylpiperazine in combination with ammonium persulfate or potassium persulfate.
Dependencies are inferred from the claim text.