Position-specific insertion vectors and method of using same
AssigneeThe Regents of the University of California
InventorSuzanne B. Sandmeyer
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Abstract
The present invention provides vectors for the efficient and position-specific integration of expressible, exogenous nucleotide sequences into cellular genomes. This invention takes advantage of the discovery of a position-specific endonuclease and position-specific insertion markers for the design of said vectors. In addition, a gene comprising a recombinant nucleic acid molecule encoding a polypeptide possessing the biological activity of a position-specific endonuclease, wherein the biological activity of said endonuclease is the catalysis of position-specific insertion of genetic material carried between the position-specific integration markers, is disclosed.
Claims7
A recombinant nucleic acid molecule comprising a gene encoding a desired protein, said gene being flanked by the position-specific integration markers of S. cerevisiae Ty3, wherein said gene is not native to Ty3.
A recombinant nucleic acid molecule comprising a gene encoding a polypeptide possessing the biological activity of S. cerevisiae Ty3 position-specific endonuclease, wherein said biological activity is the catalysis of position-specific integration of a nucleic acid sequence.
Dependencies are inferred from the claim text.