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US 5,292,657 AGranted
Process for preparing rotary disc fatty acid microspheres of microorganisms
AssigneePioneer Hi-Bred International, Inc.
InventorsWilliam M. Rutherford, Jack E. Allen, Herman W. Schlameus, Donald J. Mangold, William W. Harlowe, Jr., Joseph R. Lebeda
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Abstract
A process for preparing fatty acid microspheres of microorganisms by rotary disc processing is disclosed. The process is designed to minimize heat risk to the microorganisms during production. Microsphere particles of fatty acid matrix containing microorganisms, preferably stearic acid encapsulated Enterococcus faecium, are also provided.
Claims9
A process of preparing discrete individual fatty acid matrix microspheres of microorganisms said microorganisms dwelling in a fatty acid matrix, said process comprising:
- preparing a first feed stream of fatty acid melt of a C.sub.12 to C.sub.24 fatty acid,
- preparing a second feed stream of microorganism culture,
- feeding separately said culture feed stream and said fatty acid melt feed stream into a rotary disc processor to provide a mixture which is from 50% to about 90% by weight of said fatty acid melt, and
- rotary disc microsphere processing said mixture in a rotary disc processor to provide free-flowing fatty acid matrix microspheres of organisms.
1 more element
Dependencies are inferred from the claim text.