Method for cloning and producing the NaeI restriction endonuclease and methylase
AssigneeNew England Biolabs, Inc.
InventorsEllen P. Guthrie, Elizabeth M. Van Cott, Christopher H. Taron
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a method for cloning and producing the NaeI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia aerocolonigenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity, and 3) purifying the NaeI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity.
Claims7
Isolated DNA coding for NaeI restriction endonuclease, wherein the isolated DNA is obtainable from the plasmid pCTnaeIR16-1.
A recombinant vector comprising a vector into which DNA coding for NaeI restriction endonuclease has been inserted.
Dependencies are inferred from the claim text.