Preparation of hapalindole-related alkaloids from blue-green algae
AssigneesEli Lilly and Company, University of Hawaii
InventorsRoseanne Bonjouklian, Richard E. Moore, Gregory M. L. Patterson, Tim A. Smitka
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Abstract
New hapalindole-type alkaloids called A89271 A-F and I are antifungal and antitumor agents. Methods for preparing them and for preparing hapalindoles G and H by culturing the blue-green alga Fischerella ambigua ATCC 55210 are provided. Methods for preparing A89271 factors B and F by culturing the blue-green alga Hapalosiphon hibernicus ATCC 55225 and a biologically pure culture of this alga are also provided. Further provided are methods and compositions for the inhibition of fungal growth and the treatment of susceptible neoplasms.
Claims10
A process for preparing hapalindole-related alkaloid A89271 B that comprises culturing a blue-green alga selected from the group consisting of Fischerella ambigua ATCC 55210, Hapalosiphon hibernicus ATCC 55225, Westiellopsis prolifica Janet (UH isolate EN3-1), and a mutant thereof that produces A89271 B, under submerged aerobic conditions in a suitable culture medium until recoverable amount of A89271 B is produced, and recovering said A89271 B.
A process for producing hapalindole-related alkaloid A89271 F that comprises culturing a blue-green alga selected from the group consisting of Fischerella ambigua ATCC 55210, Hapalosiphon hibernicus ATCC 55225, and a mutant thereof that produces A89271 F, under submerged aerobic conditions in a suitable culture medium until a recoverable amount of A89271 F is produced, and recovering said A89271 F.
A process for producing hapalindole-related alkaloid A89271 factor selected from the group consisting of A89271 factors A, C, D, E and I that comprises culturing the alga Fischerella ambigua ATCC 55210, or a mutant thereof that produces the selected A89271 factor, under submerged aerobic conditions in a suitable culture medium until a recoverable amount of the selected A89271 factor is produced, and recovering said selected A89271 factor.
A process for producing a hapalindole selected from the group consisting of hapalindoles G and H that comprises culturing the alga Fischerella ambigua ATCC 55210, or a mutant thereof that produces the selected hapalindole, under submerged aerobic conditions in a suitable culture medium until a recoverable amount of the hapalindole is produced, and recovering said hapalindole.
Dependencies are inferred from the claim text.