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US 5,292,646 AGranted
Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
AssigneeGenetics Institute, Inc.
InventorsJohn McCoy, Edward R. LaVallie
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AssigneeGenetics Institute, Inc.
InventorsJohn McCoy, Edward R. LaVallie
Estimating the term…
This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
A fusion DNA comprising a first DNA encoding a thioredoxin-like protein fused in-frame to a second DNA encoding a non-euracryotic peptide or protein, said second DNA heterologous to a selected host cell.
A fusion DNA comprising a first DNA encoding a thioredoxin-like protein fused to a second DNA encoding a linker peptide.
A host cell transformed with, or having integrated into the genome thereof, a fusion DNA comprising a first DNA encoding a thioredoxin-like protein fused in-frame to a second DNA enclosing a non-eukaryotic peptide or protein heterologous to said host cell, said fusion DNA under the control of an expression control sequence.
A fusion protein comprising a thioredoxin-like protein fused to a non-E. coli .beta.-galactosidase peptide or protein.
A fusion protein comprising a thioredoxin-like protein fused to a eukaryotic peptide or protein.
A fusion protein comprising a thioredoxin-like protein fused to a linker peptide providing a cleavage site.
A method for obtaining a non-eukaryotic protein comprising the steps of:
A method for obtaining a non-eukaryotic heterologous protein comprising the steps of:
Dependencies are inferred from the claim text.