Fusaric acid resistant genes
AssigneesSuntory Limited, Daikin Industries, Ltd.
InventorsYuji Shibano, Hideyoshi Toyoda, Ryutaro Utsumi, Kazuaki Obata
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Abstract
Fusaric acid resistant genes derived from fusaric acid decomposing or detoxifying microorganisms such as Pseudomonas cepacia and Klebsiella oxytoca are described. Also described are DNA fragments and plasmids, which comprise such fusaric acid resistant genes. Host cells comprising such plasmids are also described.
Claims11
An isolated and purified DNA consisting essentially of a sequence encoding amino acid sequences which decompose or detoxify fusaric acid, said amino acid sequences being SEQ ID NOS:
- 2-6.
A process for the production of proteins of SEQ ID NOS:
- 2-6, comprising:
- transforming a host Escherichia coli cell with a plasmid containing a DNA sequence encoding said proteins;
- culturing said transformed cell in a suitable medium;
- and purifying and isolating said proteins.
An isolated and purified DNA consisting essentially of a sequence encoding amino acid sequences which decompose or detoxify fusaric acid, said amino acid sequences being SEQ ID NOS:
- 8-10.
A process for the production of proteins of SEQ ID NOS:
- 8-10, comprising:
- transforming a host Escherichia coli cell with a plasmid containing a DNA sequence encoding said proteins,
- culturing said transformed cell in a suitable medium;
- and
- purifying and isolating said proteins.
Dependencies are inferred from the claim text.