Association of bovine mitochondrial DNA with traits of economic importance
AssigneeIowa State University Research Foundation, Inc.
InventorsDonald C. Beitz, Albert E. Freeman, Michael M. Schutz, Gary L. Lindberg
Life of this patent
Estimating the term…
Abstract
A method of genetically evaluating animals by using mitochondrial DNA is disclosed. Polymorphisms in mitochondrial DNA are detected by isolating, fragmenting, and sequencing the DNA. The restriction patterns and nucleotide sequences of mitochondrial DNA of different animals are correlated to expressed traits in the animals. This may be confirmed by comparing results to expression of the trait in maternal lineages of animals. Further, effects of maternal lineages are determined by partitioning maternal genetic variation from nuclear variation.
Claims16
A method of evaluating the material mitochondrial phenotypic contribution to economic traits of milk production and reproduction efficiency of a dairy cow comprising:
- assaying for the presence of one or more genetic markers in the mitochondrial D-loop of said cow.
Genetic mitochondrial D-Loop markers for milk production and reproductive efficiency traits in dairy cattle, said markers comprising the group consisting essentially of:
- a transition at mitochondrial D-loop position 8, 106, 169, 16022, 16049, 16057, 16058, 16074, 16085, 16113, 16141, 16230, 16231, 16247, 363 or 16111.
A method of evaluating inheritable milk production and reproduction efficiency traits in dairy cattle by partitioning effects of mitochondrial lineages from nuclear effects, said method comprising:
- isolating mitochondrial DNA from a group of said dairy cows,
- fragmenting and sequencing said mitochondrial DNA,
- comparing said mitochondrial DNA of said group of dairy cows to known mitochondrial sequences to detect nucleotide sequence polymorphism,
- determining the presence of said polymorphic mitochondrial DNA sequences with a milk production phenotypic trait of said dairy cow, and
- selecting said cattle for favorable breeding features.
Dependencies are inferred from the claim text.