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US 5,292,507 AGranted
Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
AssigneeImperial Oil Limited
InventorRobert Charley
Life of this patent
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Abstract
Soluble, non-crosslinked polysaccharides are used to stabilize microorganisms for use as inoculants in agriculture. Preferably, the polysaccharide is alginate and plant seeds are inoculated. A solution of the polysaccharide and a suspension of the microorganism are mixed to form a composition containing about 0.005% to about 10% of the polysaccharide. The composition can be stored for one week or more before use and the composition may be dried.
Claims12
A method for maintaining a microorganism comprising the steps of:
- (i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth or yield of a plant;
- (ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking;
- (iii) preparing a suspension of said microorganism;
- (iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4.degree. C.-30.degree. C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide;
- and
- (v) storing the composition.
3 more elements
A method for inoculating a plant seed with a microorganism comprising the steps of:
- (i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth of yield of a plant;
- (ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking;
- (iii) preparing a suspension of said microorganism;
- (iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4.degree. C.-30.degree. C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide;
- and
- (v) storing the composition;
- (vi) contacting the plant seed with said composition;
- and
- (vii) allowing said composition to inoculate the plant seed.
6 more elements
Dependencies are inferred from the claim text.