Detecting a radiation signal
AssigneeBeckman Instruments, Inc.
InventorWilbur I. Kaye
Life of this patent
Estimating the term…
Abstract
Focusing a generated radiation signal on a detector is effected by a curvilinear surface. The radiation beam signal from the reflected surface is collimated or focused on the detector. Light scattered, Raman scattered, fluorescence, chemiluminescence, phosphorescence radiation signals from particles as a result of a chemical procedure or reaction is enhanced through this focusing technique. The enhanced signal which is detected is subsequently measured through different detection techniques.
Claims52
Apparatus for directing a radiation signal to a detector comprising means for generating an incident radiation signal caused by the interaction of a flowing sample with an exciting radiation source, reflection means being a reflecting surface of revolution having an axis and having a focal point, for receiving the generated incident radiation signal and for reflecting the incident signal as an output reflection signal to a detector, means for generating the incident radiation signal being substantially at the focal point of the reflection means, wherein at least one of the directions of the flowing sample or the direction of exciting radiation is substantially collinear with the axis of the reflecting surface at least at the focal point, means for flowing a sample substantially along the axis of revolution, said means being an elongated tube having a side wall, and wherein the exciting radiation source is a laser radiation such that the laser radiation essentially travels within the tube and is free of contacting the tube side wall.
Apparatus for directing a light scattered signal to a detector comprising reflecting means being a reflecting surface of revolution for receiving an incident light scattered signal, a focal point for the reflecting surface, a detector spaced from the reflecting surface, the reflecting means being for reflecting the light scattered signal to the detector, means for generating a laser radiation beam along an axis of the reflecting surface, means for directing a sample coaxially with the reflecting surface, such that the laser radiation beam and the sample impact at least at the focal point, means for directing the light scattered signal from the focal point of the reflecting surface such that the incident light scattered signal is directed from the reflecting surface towards the detector.
Apparatus for directing a radiation signal to a detector comprising reflection means being a reflecting surface of revolution having an axis and a focal point for receiving an incident radiation signal, a detector spaced from the reflecting surface, the reflecting means being for reflecting the incident radiation signal as a reflection signal to the detector, means for generating an excitation signal beam, a tube for flowing a sample, means for directing the excitation signal beam to impact a sample flow along the axis of radiation means at the focal point thereby generating the incident radiation signal substantially at the focal point such that the incident radiation signal is directed from the focal point over a solid angle in the range of about 45.degree. to 135.degree. relative to the axis of revolution, and optical occlusion means for optically occluding the tube from generating the incident radiation, the occlusion means about the tube defining barrier means, the barrier means radiating relative to the focal point to define the solid angle.
Apparatus for directing a radiation signal to a detector comprising a substantially curvilinear reflecting surface having a focal point and an axis of revolution, a detector, means for generating an incident radiation signal along the axis of revolution by the interaction of a sample with an excitation signal at the focal point, the sample being directed along the axis of revolution, means for directing the generated incident radiation signal to the reflecting surface, the reflecting surface reflecting an output signal to the detector, and including means for responding selectively to a spatial or temporal sorted signal, a Raman scatter signal.
Apparatus for directing a radiation light scattered signal to a detector comprising a curvilinear reflecting surface having a focal point and an axis of revolution, the reflecting surface being for receiving a generated incident radiation signal, means for directing a flowing sample axially relative to the axis of revolution towards the focal point, a detector, the reflecting surface transmitting a reflected signal to the detector, means for generating an incident radiation signal by the interaction of a sample with an excitation signal directed axially relative to the axis of revolution, including means for selecting the excitation radiation signal to have a wavelength greater than a particle size in the sample whereby the incident radiation reflected from the particles is isotropic.
A method for directing a radiation signal to a detector comprising generating an incident radiation signal by the interaction of a sample with an excitation source, the sample and radiation from the excitation source being directed coaxially relative reflection means being a curvilinear reflecting surface of revolution source, receiving the generated incident radiation signal from a focal point of the reflecting surface, generating an output reflected signal from the reflecting surface, and directing the reflected signal to a detector.
A method for directing a light scattered signal to a detector comprising generating a laser radiation beam along an axis of a curvilinear reflecting surface having a focal point, directing a sample axially to the focal point whereby the laser radiation beam impacts the sample at the focal point, and directing a light scattered generated signal from an impact at the focal point to the curvilinear reflecting surface and reflecting the incident light scattered signal towards a detector.
A method for directing a radiation signal to a detector, the signal being selected from the group consisting of a light scattered signal, Raman scattering, luminescence, phosphorescence and fluorescent signal, comprising generating an excitation signal beam and directing the excitation signal beam to a sample to generate an incident radiation signal, receiving a radiation signal on a curvilinear reflecting surface, the curvilinear surface having an axis of revolution and a focal point and being for reflecting the incident radiation signal from the focal point towards a detector, the sample and the excitation signal being coaxially directed relative to the axis of revolution and being directed towards each other at the focal point at a selected angle relative to each other, and wherein the generated radiation signal from the impact is generated at the focal point such that a generated radiation signal is directed to the curvilinear surface and in turn, towards the detector.
A method for directing a radiation signal to a detector comprising generating the radiation signal by the interaction of a sample with an excitation signal at a focal point of curvilinear reflecting means, the radiation signal and flow of the sample being coaxial, directing the generated signal as an incident radiation signal to the reflecting means, reflecting the incident signal to a detector and including having the detector respond selectively to a spatially or temporally sorted reflected signal.
Apparatus for directing a radiation signal to a detector comprising means for generating an incident radiation signal caused by the interaction of a flowing sample with an exciting radiation source, reflection means being a reflecting surface of revolution having an axis and having a focal point, and being for receiving the generated incident radiation signal and for reflecting the incident signal as an output reflection signal to a detector, and the means for generating the incident signal being at substantially the focal point, and means for flowing a sample wherein the flowing sample and the exciting radiation source are directed in a substantially collinear direction.
Apparatus for directing a light scattered signal to a detector comprising reflecting means being a reflecting surface of revolution for receiving an incident light scattered signal, the reflecting surface having a focal point, a detector spaced from the reflecting surface, the reflecting means being for reflecting the light scattered signal to the detector, means for generating a laser radiation beam, means for directing a sample into the area of the reflecting surface such that the laser radiation beam and the sample impact at least at the focal point, means for directing the light scattered signal from the focal point of the reflecting surface such that the incident light scattered signal is directed from the reflecting surface towards the detector, and wherein the means for directing the sample includes an elongated tube and wherein the laser radiation enters the sample through a window upstream of the tube, and the sample travels along a coaxial path upstream of a mouth to the elongated tube such that only laser radiation substantially axially directed and free of light scattered by the window when the laser radiation enters the sample flow passes through the tube.
Apparatus for directing a radiation signal to a detector comprising reflecting means for receiving an incident radiation signal, the reflecting means being a reflecting surface of revolution having an axis and a focal point, a detector spaced from the reflecting surface, the reflecting surface being for reflecting the incident radiation signal to the detector, means for generating an excitation signal beam, means for directing a sample flow coaxial with the axis, means for directing an excitation beam to impact the sample flow along the axis at the focal point thereby generating the incident radiation signal substantially at the focal point of the reflecting surface such that the incident radiation signal is directed from the focal point over a solid angle in the range of about 45.degree. to 135.degree. relative to the axis.
A method for directing a radiation signal to a detector comprising generating an incident radiation signal by the interaction of a sample with radiation from the excitation source, the sample and radiation from the excitation source being directed coaxially, receiving the generated incident radiation signal from a focal point of a reflecting surface, generating an output reflected signal from the reflecting surface, and directing the reflected signal to a detector.
A method as claimed in 43 wherein the generated radiation defines a solid angle in the range of about 45.degree. to 135.degree. relative to the axis of revolution.
A method for directing a light scattered signal to a detector comprising generating a laser radiation beam to a focal point of a curvilinear reflecting surface, directing a sample axially to the focal point of the curvilinear reflecting surface along an axis of the curvilinear surface whereby the laser radiation beam impacts the sample at the focal point, directing a light scattered generated signal from an impact at the focal point to the curvilinear reflecting surface, and reflecting the incident light scattered signal towards a detector.
A method for directing a radiation signal to a detector, the signal being selected from the group consisting of a light scattered signal, Raman scattering, luminescence, phosphorescence and fluorescent signal, comprising generating an excitation signal beam and directing the excitation signal beam to a flowing sample to generate an incident radiation signal, receiving a radiation signal on a curvilinear reflecting surface, the curvilinear surface having an axis of revolution and a focal point and being for reflecting the incident radiation signal from the focal point towards a detector, the sample flow and the excitation signal being coaxial and being directed towards each other at the focal point at a selected angle relative to each other, and wherein the generated radiation signal from the impact is generated at the focal point such that a generated radiation signal is directed to the curvilinear surface and in turn, towards the detector.
A method for directing a radiation signal to a detector comprising generating the radiation signal by the interaction of a flowing sample with an excitation signal directed coaxially with the sample, the radiation signal being generated at a focal point of a curvilinear reflecting surface, directing the generated signal as an incident radiation signal to the reflecting surface, reflecting the incident signal to a detector, and including having the detector respond selectively to a spatially or temporally sorted reflected signal.
Apparatus for directing a light scattered signal to a detector comprising reflection means being a reflecting surface of revolution for receiving an incident light scattered signal, the reflecting surface having a focal point, a detector spaced from the reflecting surface, means for generating a laser radiation beam, means for directing a sample into the area of the reflecting surface such that the laser radiation beam and the sample impact at least at the focal point, means for directing the light scattered signal form the focal point of the reflecting surface such that the incident light scattered signal is directed from the reflecting surface towards the detector, and wherein the means for directing the sample includes an elongated tube and wherein the laser radiation enters the sample through a window upstream of the tube, and the sample travels along a coaxial path upstream of a mouth to the elongated tube such that only laser radiation substantially axially directed and free of light scattered by the window when the laser radiation enters the sample flow passes through the tube.
Apparatus for directing a radiation signal to a detector, the signal being caused by an interaction of a sample flow with an exciting radiation source, the apparatus comprising reflection means being a reflecting surface of revolution having an axis and having a focal point, and being for receiving a generated incident radiation signal and for reflecting the incident signal as an output reflection signal to a detector, the means for generating the incident radiation signal being substantially at the focal point, an elongated tube for a sample flow, the tube having a direction, such direction of the tube being substantially along the axis, and the exciting radiation source having a direction, such exciting radiation source direction being substantially collinear with the axis at least at the focal point.
Dependencies are inferred from the claim text.