Two-dimensional gel electrophoresis apparatus
AssigneeShimadzu Corporation
InventorShin Nakanura
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a two-dimensional electrophoresis apparatus comprising a first-dimensional electrophoresis part (5), a second-dimensional electrophoresis part (6) and a transcription part (7). The transcription part (7) transfers a first-dimensional gel completely electrophoresed by the first-dimensional electrophoresis part (5) from a first-dimensional gel housing (20) to one end of a second-dimensional gel housing (30). Thus, a two-dimensional electrophoresis can be automatically executed, thereby improving reproducibility of separated images and separative power.
Claims13
A two-dimensional gel electrophoresis apparatus, comprising:
- a first-dimensional gel housing storing a first-dimensional gel;
- first-dimensional electrophoresis means for conducting a first-dimensional electrophoresis with said first-dimensional gel;
- a second-dimensional gel housing storing a second-dimensional gel;
- second-dimensional electrophoresis means for conducting a second-dimensional electrophoresis with said second-dimensional gel;
- and
- gel transfer means for transferring said first-dimensional gel after being electrophoresed by said first-dimensional electrophoresis means from said first-dimensional gel housing to one end of said second-dimensional gel housing, said gel transfer means comprising:
- a grasping mechanism for grasping said first-dimensional gel housing,
- a moving mechanism for raising up a lower end of said first-dimensional gel housing to a position higher than an upper end of said second-dimensional gel housing and horizontally moving said first-dimensional gel housing longitudinally along the upper end of said second-dimensional gel, and
- an extrusion mechanism for extruding said first-dimensional gel from said first-dimensional gel housing following said horizontal movement of said first-dimensional gel housing by said moving mechanism at the same speed as said horizontal movement.
A two-dimensional gel electrophoresis apparatus, said apparatus comprising:
- a first-dimensional gel housing storing a first-dimensional gel;
- first-dimensional electrophoresis means for conducting a first-dimensional electrophoresis with said first-dimensional gel;
- a second-dimensional gel housing storing a second-dimensional gel;
- second-dimensional electrophoresis means for conducting a second-dimensional electrophoresis with said second-dimensional gel;
- and
- gel transfer means for transferring said first-dimensional gel after being electrophoresed by said first-dimensional electrophoresis means from said first-dimensional gel housing to one end of said second-dimensional gel housing, said gel transfer means including means for positioning and moving said first-dimensional gel housing adjacent one end of said second-dimensional gel housing and means for extruding said first-dimensional gel along one end of said second-dimensional gel while moving said first-dimensional housing.
A two-dimensional gel electrophoresis apparatus, said apparatus comprising:
- a first-dimensional gel housing;
- a first-dimensional gel disposed within said first-dimensional gel housing;
- a second-dimensional gel housing;
- a second-dimensional gel disposed within said second-dimensional gel housing;
- an extruding device for extruding said first-dimensional gel from said first-dimensional gel housing directly along one end of said second-dimensional gel after said first gel has been electrophoresed.
Dependencies are inferred from the claim text.