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US 5,290,924 AGranted
Recombinant promoter for gene expression in monocotyledonous plants
OwnerDavid I. Last, Richard I. S. Brettell +7inventors
InventorsDavid I. Last, Richard I. S. Brettell, Douglas A. Chamberlain, Philip J. Larkin, Ellen L. Marsh, James W. Peacock, Elizabeth S. Dennis, Mark R. Olive, Jeffrey G. Ellis
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Abstract
A recombinant promoter molecule for enhancing expression of a plant-expressible structural gene in a monocot plant cell is provided comprising a plurality of enhancer elements selected from the group consisting of ARE and OCS elements, a truncated plant-expressible promoter, and an intron.
Claims18
A recombinant promoter molecule, useful for enhancing expression of a plant-expressible structural gene in a monocot plant cell, said promoter molecule comprising:
- (a) a plurality of enhancer elements selected from the group consisting of only ARE elements, only OCS elements, and combinations of ARE and OCS elements;
- (b) a truncated, plant expressible promoter, providing a TATA box region and a transcription start site, said promoter selected from the group consisting of .DELTA.35S and .DELTA.ADH positioned 3' to said plurality of enhancer elements wherein said truncated promoter excludes the presence of enhancer sequences and wherein said truncated promoter is recombined with said plurality of enhancer elements positioned 5' to said truncated promoter;
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- (c) a maize Adh1 intron positioned 3' to said transcription start site whereby a plant-expressible structural gene, placed 3' to said recombinant promoter molecule, is expressed in said monocot plant cell under regulatory control of said recombinant promoter molecule.
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Dependencies are inferred from the claim text.