Method of purifying human epidermal growth factor
AssigneeAllelix Biopharmaceuticals Inc.
InventorsDennis R. Sindrey, Chandra M. Dwivedi
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Abstract
Human epidermal growth factor is provided in an ultrapure form characterized by the absence of protein contaminants detectable by capillary electrophoresis analysis. A method for obtaining such ultrapure human epidermal growth factor includes fractionation of a human epidermal growth factor preparation by reversed phase high performance liquid chromatography in the presence of a cationic ion-pairing agent.
Claims12
A method for purifying human epidermal growth factor, which comprises the step of fractionating a human epidermal growth factor preparation by reversed phase high performance liquid chromatography in the presence of an amine-based cationic ion-pairing agent or a salt thereof.
A method for obtaining essentially pure human epidermal growth factor, comprising the steps of
- i) obtaining a human epidermal growth factor preparation;
- ii) fractionating said preparation by reversed phase high performance liquid chromatography in the presence of triethylamine or a salt thereof;
- and
- iii) collecting, following the chromatographic step, essentially pure human epidermal growth factor.
Dependencies are inferred from the claim text.