Hemoglobin intramolecularly cross-linked with trivalent reagents
AssigneeUniversity of Maryland, Baltimore
InventorsEnrico Bucci, Clara Fronticelli
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Abstract
Stroma-free hemoglobin cross-linked with reagents that mimic 2,3-diphosphoglycerate and transform stroma-free hemoglobin into a physiologically competent oxygen carrier which is retained in vivo for adequate periods of time, and thus can be used in fluids for transporting oxygen is described.
Claims30
Stroma-free hemoglobin intramolecularly cross-linked with a compound of the following formula (I):
- ##STR3## wherein R is a leaving atom or group capable of activating the carboxyl moiety to which it is attached and X is a member selected from the group consisting of a trivalent C.sub.1-12 aliphatic group, a trivalent C.sub.6 aromatic group, a trivalent C.sub.5-6 heterocyclic group and a fused ring group.
The stroma-free hemoglobin as claimed in claim wherein said trivalent aliphatic group is selected from the group consisting of a C.sub.1-12 alkyl group, a C.sub.2-12 alkenyl group, a C.sub.3-12 alkynyl group and a C.sub.3-12 alicylic group.
The stroma-free hemoglobin as claimed in claim wherein said C.sub.5-6 heterocyclic group is selected from the group consisting of pyrrolyl, furanyl, thiophenyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyridinyl and pyrimidinyl.
The cross-linked stroma-free hemoglobin as claimed in claim wherein said stroma-free hemoglobin is bovine hemoglobin.
A method for producing stroma-free intramolecularly cross-linked hemoglobin comprising cross-linking stroma-free hemoglobin with a compound of the following formula (I):
- ##STR4## wherein R is a leaving atom or group capable of activating the carboxyl moiety to which it is attached and X is a member selected from the group consisting of a trivalent C.sub.1-12 aliphatic group, a trivalent C.sub.6 aromatic group, a trivalent C.sub.5-6 heterocyclic group and a fused ring group.
Dependencies are inferred from the claim text.