Method of immunoassay measurement

A method of immunoassay measurement comprising the steps of:

• preparing a cartridge having a plurality of wells, at least a first well of said plurality of wells being deeper than the other wells and containing solid phase material carrying antigen or antibody, a second well being empty and a third well being filled with labeled antigen or antibody solution;
• aspirating a sample and a portion of said labelling or antibody solution from said third well into said second well;
• stirring the labeled antigen or antibody and the sample in said second well;
• aspirating and pouring a portion of the mixture in said second well into said at least a first one of said plurality of wells;
• stirring the mixture of the labeled antigen or antibody, the sample and solid phase material carrying antigen or antibody in said first well and then allowing an antigen-antibody reaction to occur;
• performing a bind/free separation in a portion of said first well protruding downwardly from said second and third wells;
• washing out free substance containing non-reacted labelling antigen or antibody in said portion of said first well;
• adding substrate to said at least a first one of said plurality of wells and stirring a mixture of the solid phase material and the substrate therein;
• and
• measuring a signal obtained by reaction of label bound on solid phase material the substrate through said portion of said at least a first one of said plurality of wells.

A method of immunoassay measurement comprising the steps of:

• preparing a cartridge having a plurality of wells, at least a first well of said plurality of wells being deeper than the other wells and containing solid phase material carrying antigen or antibody and at least one of said the other wells being filled with labeled antigen or antibody solution;
• pouring a sample into said at least a first one of said plurality of wells;
• stirring a mixture of the solid phase material carrying antigen or antibody and the sample in said at least a first one of said plurality of wells and then allowing an antigen-antibody reaction to occur therein;
• performing a bind/free separation in a portion of said at least a first one of said plurality of wells protruding downwardly from said the other wells;
• washing out non-reacted, free substance;
• aspirating a portion of the labeled antigen or antibody solution in one of the other wells and pouring said portion into said first well;
• stirring a mixture of the solid phase material and the portion of the labeled antigen or antibody in said at least a first one of said plurality of wells and then allowing an antigen-antibody reaction to occur therein;
• performing a bind/free separation in a portion of said first well protruding downwardly from the other wells;
• washing out free substance containing non-reacted labeled antigen or antibody;
• adding substrate to said at least a first one of said plurality of wells and stirring a mixture of the solid phase material and the substrate therein;
• and
• measuring a signal obtained by reaction on label bound on solid phase material with the substrate through said portion of said at least a first one of said plurality of wells.