Back to results
US 5,290,691 AGranted
Safe and versatile cloning vector
AssigneeBanyu Seiyaku Kabushiki Kaisha
InventorsSusumu Mitsuhashi, Matsuhisa Inoue, Masao Nagashima
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeBanyu Seiyaku Kabushiki Kaisha
InventorsSusumu Mitsuhashi, Matsuhisa Inoue, Masao Nagashima
Estimating the term…
A safe and versatile recombinant DNA cloning vector having at least two functionally different replication origins selected independently from the group consisting of a replication origin functional in Bacillus subtilis, a replication origin functional in Escherichia coli and a replication origin functional in Pseudomonas aeruginosa, and at least one DNA segment which provides resistance against at least one antibiotic after introduction into susceptible host cells. The origin for replication origin functional in Bacillus subtilis can be a restriction fragment of a plasmid of Gram positive bacteria, and that Escherichia coli or Pseudomonas aeruginosa can be a restriction fragment of a plasmid of Gram negative bacteria, e.g. a restriction fragment of plasmids pMS 140-1 or pMS 71.
Versatile recombinant DNA cloning vector having a first replication origin functional in Escherichia coli and Pseudomonas aeruginosa, and a second replication origin functional in Bacillus subtilus, said versatile recombinant DNA cloning vector also having genes expressing resistance to two types of antibiotic in each of said three species of microorganisms, after introduction into transformable host cells of said microorganisms which are sensitive to antibiotics, said vector being non-mobilizable in E. coli cells with coexisting transferable plasmids or together with other non-transferable plasmids.
A versatile recombinant DNA cloning vector pMS 504 (Ap.sup.r Tc.sup.r).
A versatile recombinant DNA cloning vector pMS 505-1 (Ap.sup.r Sm.sup.r).
Dependencies are inferred from the claim text.