Method for separation and concentration of phosphopeptides
AssigneeMeiji Milk Products Co., Ltd.
InventorsKaoru Koide, Takashi Fukushima, Tamotsu Kuwata, Koki Itoyama, Fumio Miyazawa
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for separating and concentrating acidic peptide, especially phosphopeptide having a phosphoserine residue, from a peptide mixture prepared by digesting casein with protease.
Claims12
A method for separating phosphopeptides from an aqueous solution containing casein peptides, wherein said aqueous solution was prepared by treating casein with an enzyme selected from the group consisting of trypsin, pepsin, chyrotrypsin and papain, comprising:
- (a) adjusting the pH of the aqueous solution of casein peptides to a pH value of from 1.5 to 5.0;
- (b) contacting the aqueous solution of casein peptides with a crosslinked chitosan molding and adsorbing said casein peptides onto said crosslinked chitosan molding;
- (c) desorbing phosphopeptides from said crosslinked chitosan molding under pH conditions of less than 1.5 or more than 5.0 to obtain a solution containing phosphopeptides;
- and
- (d) concentrating the solution containing phosphopeptides obtained in step (c) to give a concentrated solution of phosphopeptides and optionally spray-drying further said concentrated solution to form powdered phosphopeptides.
A method for separating phosphopeptides from an aqueous solution containing casein peptides, wherein said aqueous solution was prepared by treating casein with an enzyme selected from the group consisting of trypsin, pepsin, chymotrypsin and papain, consisting essentially of:
- (a) adjusting the pH of the aqueous solution of casein phosphopeptides to a pH value of from 1.5 to 5.0;
- (b) contacting the aqueous solution of casein peptides with a crosslinked chitosan molding and adsorbing said casein peptides onto said crosslinked chitosan molding;
- (c) desorbing phosphopeptides from said crosslinked chitosan molding under pH conditions of less than 1.5 or more than 5.0 to obtain a solution containing phosphopeptides;
- and
- (d) concentrating the solution containing phosphopeptides obtained in step (c) to give a concentration solution of phosphopeptides, and optionally further spray-drying said concentration solution to form powdered phosphopeptides.
Dependencies are inferred from the claim text.