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US 5,290,683 AGranted
Rapid analysis of ethanol in body fluids
OwnerYedy Israel, Bhushan Kapur +1inventors
InventorsYedy Israel, Bhushan Kapur, Guang-chou Tu
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OwnerYedy Israel, Bhushan Kapur +1inventors
InventorsYedy Israel, Bhushan Kapur, Guang-chou Tu
Estimating the term…
An assay for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprises a solid carrier. The carrier is impregnated with a solution having the following constituents mixed in effective amounts: a cross-linked stabilized enzyme for oxidizing the alcohol in the presence of a reducible compound to produce a corresponding aldehyde and a reduction product, a chromogen which reacts with the reduction product in the presence of an agent to produce a colored compound indicative of alcohol presence in the biological fluid, a competitive inhibitor of the enzyme, an agent for converting the chromogen to the colored compound, and a buffer to maintain the solution at a predetermined acidic pH.
A composition for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprising a solid carrier impregnated with a solution having the following constituents mixed in effective amounts,
A composition for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprising a solid carrier impregnated with a solution having the following constituents mixed in respective amounts:
Dependencies are inferred from the claim text.