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US 5,290,682 AGranted
Enzyme controlled processes and products
AssigneePolaroid Corporation
InventorsFrank A. Meneghini, Paul S. Palumbo
Life of this patent
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Abstract
Compounds which are useful as substrates for enzymes are disclosed. An enzyme-controlled process is provided for generating a colored species from an initially substantially colorless material as a result of enzymatic attack. The process can be exploited to provide an enzyme-amplified diagnostic assay method to detect an analyte of interest present in a test sample.
Claims6
A process for converting an initially substantially colorless compound to a colored species comprising
- reacting a substantially colorless compound represented by the formula ##STR7## wherein W, X, Y and Z are the same or different and each is represented by --CH.sub.2).sub.a (CHR.sub.4)(CH.sub.2 --.sub.b ;
- D is a moiety which can be oxidized by an enzyme to a more electron deficient species;
- R, R.sub.1, R.sub.2, R.sub.3, R.sub.4 each independently is hydrogen, alkyl or aryl;
- Q is --CO or --SO.sub.2 ;
- a and b each independently is an integer of from 0 to 4, provided that the sum of a+b is an integer of from 1 to 4;
- and
- m, n, p, q, w, x, y and z each independently is 0 or 1, provided that the sum of m+n+w+x is 2 and the sum of p+q+y+z is 2;
- with an enzyme or an enzyme labeled antigen or antibody in the presence of hydrogen peroxide to form a colored species, wherein said enzyme or said enzyme labeled antigen or antibody oxidizes said oxidizable moiety D to a more electron deficient species.
5 more elements
Dependencies are inferred from the claim text.