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US 5,290,681 AGranted
Method of assaying immunization stimulating activity
AssigneeMorinaga & Co., Ltd.
InventorsKazuhiko Kuroda, Hiroki Murakami, Shuichi Hashizume
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Abstract
A method of assaying immunization stimulating activity comprising (a) immunizing a lymphocyte in vitro by culturing the lymphocyte in contact with an immobilized antigen in a medium containing a test substance and (b) detecting an immunoglobulin, which is produced and secreted into the medium by the immunized lymphocyte and bound to the immobilized antigen, by counting the immunostained spots deposited in situ on the surface of the immobilized antigen.
Claims5
A method of assaying immunization stimulating activity comprising
- (a) immunizing a lymphocyte in vitro by culturing the lymphocyte in contact with immobilized cancer cells in a medium containing an adjuvant, a lymphokine and a test substance in the absence of a polyclonal actinator, said test substance not being said adjuvant or said lymphokine,
- (b) adding labeled anti-immunoglobulin antibody and
- (c) detecting an immunoglobulin, which is produced and secreted into the medium by the immunized lymphocyte and bound to the immobilized cancer cell, by counting immunostained spots deposited in situ on the surface of the immobilized cancer cell.
Dependencies are inferred from the claim text.