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AssigneeTakeda Chemical Industries, Ltd.
InventorsKoichi Kondo, Takahisa Kobayashi, Makoto Katsuno
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The present invention relates to a monoclonal antibody which specifically recognizes 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha. with high sensitivity, a hybridoma producing said monoclonal antibody, a method of establishing said hybridoma and an immunoassay method for 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha. using said monoclonal antibody. The monoclonal antibody of the present invention makes it possible to conveniently measure 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha., a stable metabolite of prostaglandin I.sub.2 thought of as reflecting the concentration changes therein, with specificity and high sensitivity. It is useful in determining the content of prostaglandin I.sub.2, which exhibits platelet aggregating inhibitory activity, vasodilating activity and other activities, in biological samples.
A monoclonal antibody reacting specifically to 6,15-diketo-13,14-dihydro-prostaglandin F.sub.1 .alpha., wherein said antibody does not significantly react with 6-keto-PGF.sub.1 .alpha., 2,3-dinor-6-keto-PGF.sub.1 .alpha., 6-keto-PGE.sub.1, PGB.sub.2, PGD.sub.2, PGE.sub.1, PGE.sub.2, PGF.sub.1 .alpha., PGF.sub.2 .alpha., TXB.sub.2, 11-dehydro-TXB.sub.2, 15-keto-PGF.sub.2 .alpha., 13,14-dihydro-15-keto-PGE.sub.1 or 13,14-dihydro-15-keto-PGF.sub.2 .alpha..
Dependencies are inferred from the claim text.