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AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsBetty H. Robertson, Omana V. Nainan, Vicki K. Brown, Harold S. Margolis, Bhawna Khanna
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Provided are methods for detecting hepatitis A virus (HAV) and HAV genotypes I and III by capturing whole hepatitis A virus with antibodies specific to hepatitis A virus, generating a cDNA copy of the RNA by reverse transcription in the presence of a negative strand primer having the sequence defined by SEQ ID NO:1, amplifying the cDNA by polymerase chain reaction with both the negative strand primer and a labelled or unlabelled positive strand primer having the sequence defined by SEQ ID NO: 2, and detecting the amplified cDNA by hybridization with either or both of the probes defined by SEQ ID NO:3 and SEQ ID NO:4 or by detection of label, wherein the presence of detectable hybridization or amplification indicates the presence of hepatitis A virus. Also provided are DNA primers and probes from the amino-terminal portion of the VPI region of the HAV genome that selectively hybridize with HAV, HAV genotype I and HAV genotype III and can amplify the amino-terminal portion of the VP1 region of HAV.
A method for detecting hepatitis A virus comprising the steps of:
A method for detecting Genotype I of hepatitis A virus comprising the steps of:
A method for detecting Genotype III of hepatitis A virus comprising the steps of:
A DNA primer that hybridizes with hepatitis A virus, consisting of the sequence defined by SEQ ID NO:1.
DNA primer that hybridizes with hepatitis A virus, consisting of the sequence defined by SEQ ID NO:
A DNA probe that hybridizes with hepatitis A virus genotype I, consisting of the sequence defined by SEQ ID NO:3.
A DNA probe that hybridizes with hepatitis A virus genotype III, consisting of the sequence defined by SEQ ID NO:4.
Dependencies are inferred from the claim text.