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US 5,290,536 AGranted
Phenyl substituted 2-thiazolyl tetrazolium salt indicators
AssigneeMiles Inc.
InventorsJurgen Kocher, Klaus Wehling
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Abstract
Phenyl substituted 2-thiazolyl tetrazolium salt compounds characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic indicators for reducing substances such as NADH. The reflectance plateau confers improved accuracy to analytical assays, particularly for the determination of analytes of medical diagnostic significance, in which a colorimetric response on a reagent carrier matrix is measured by reflectance.
Claims17
Phenyl substituted 2-thiazolyl tetrazolium salt indicators characterized by the formula:
- ##STR11## wherein R.sup.1 and R.sup.2 are such that the 2-thiazolyl group is 4,5-bis(4-methoxyphenyl)thiozol-2-yl;
- 4,5-diphenylthiazol-2-yl;
- 4-phenylthiazol-2-yl;
- 4-phenyl-5-methylthiazol-2-yl or 4-naphthyl-5-phenylthiazol-2-yl;
- wherein R.sup.4 is 4-carboxyphenyl;
- phenyl;
- 3-pyridyl;
- 4-nitrophenyl;
- 3,5-dicarboxyphenyl;
- 4-nitrophenyl;
- 8-quinolyl;
- or 3,4,5-trimethoxyphenyl;
- wherein R.sup.3 is 3-4-methylenedioxyphenyl;
- 4-methoxyphenyl;
- 2-thienyl, 4-fluorophenyl;
- or 3-thienyl and X.sup..crclbar. is a counteranion and wherein the compounds are further characterized in that the reflectance spectra exhibited by each of said compounds when reduced to its colored formazan state varies by less than about 17% over a wavelength range of 50 nm when the midpoint of the 50 nm range is 640 nm or greater.
13 more elements
Dependencies are inferred from the claim text.