US 5,290,419 A · Fluorescence detection type electrophoresis apparatus

Abstract

In the multi-color fluorescence detection type electrophoresis apparatus provided with the electrophoresis gel plate, excitation laser source, means to separate the fluorescence images according to each emission wavelength, and the detector of the fluorescence subjected to wavelength selection, two or more laser sources are provided, each of laser lights is irradiated on the sample on a time-sharing basis, and the filter which cuts off the scattered light of each the laser synchronous with the laser beam is installed in front of the wavelength separation means thereby providing simultaneous, quick and real-time analysis of a great number of samples such as DNA and RNA labeled by many types of fluorophores, without overlapping the wavelengths of the excitation light and the fluorescence.

Claims9

Dependent claims

In the electrophoresis apparatus comprising

i) means for electrophoretically separating a group of samples composed of the samples labeled by a plurality types of fluorophores,
ii) excitation means for exciting the fluorophores labeling said samples and emitting fluorescence,
iii) means for selecting wavelength of said fluorescence, and
iv) means for detecting said fluorescence subjected to wavelength selection;
a fluorescence detection type electrophoresis apparatus characterized in that said excitation means provides time-shared irradiation of the samples by a plurality of excitation lights, and said means for selecting wavelength has scattered light cut off filters synchronous with the time-shared irradiation of said plurality of excitation lights.

2 more elements

Dependencies are inferred from the claim text.

Fluorescence detection type electrophoresis apparatus

Life of this patent

Fluorescence detection type electrophoresis apparatus

Life of this patent

Abstract

Claims9