Cell specific gene regulators
AssigneeThe General Hospital Corporation
InventorsThomas Quertermous, Mu-En Lee
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Abstract
Methods and compositions for cell-specific expression of genes are provided. The invention utilizes the endothelin-1 promoter and promoter elements to direct expression of heterologous genes in endothelial cells. The promoters and elements are useful for directing cell-specific expression of therapies for vascular and cardiovascular diseases.
Claims15
An isolated DNA molecule comprising an element of the endothelin-1 (ET-1) promoter (SEQ ID No:1) capable of directing high-level cell-specific expression of a heterologous gene in endothelial cells operably linked to a heterologous gene.
A method for directing expression of a gene of interest in vascular endothelial cells, said method comprising:
- forming a DNA construct wherein a DNA molecule comprising an element of the endothelin-1 (ET-1) promoter[SEQ ID NO:1] is operably linked to said gene;
- and
- transforming endothelial cells with said DNA construct.
Dependencies are inferred from the claim text.