US 5,288,708 AGranted

Pharmacologically active substance BPC, the process for its preparation and its use in the therapy

OwnerPredrag Sikiric, Marijan Petek +7inventors

InventorsPredrag Sikiric, Marijan Petek, Ivo Rotkvic, Stjepan Mise, Simun Krizanac, Ivan Udovicic, Ernest Suchanek, Marko Duvnjak, Jerka Jukic

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Abstract

A new substance BPC; the process for its preparation from human and animal gastric juice comprising purifications steps: centrifugalization, dialysis, ion-exchange, chromotagraphy, gal chromatography and lyophilisation; a drug for human and veterinary use and a process for the treatment of stress-induced diseases and disorders, inflammation and edemas, diseases and disorders of dopaminergic etiology, for treatment of diabetes mellitus, diseases and disorders of gastrointestinal tract, heart, kidney, pancreas, liver, testis, hematopoetic, skeletal and nervous, system, dementias, wound, burns, fracture, viral diseases, malignancy; in surgery; some disorders of fertility and immune system; protection against ionizing radiation; for veterinary use in commercial breeding.

Claims4

Dependent claims

A process for preparation of the substance BPC having a molecular weight 40000.+-.5000 Dal and partial N-terminal amino acid sequence:

H.sub.2 N-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val--. . . --COOH from human or animal gastric juice characterized with following production steps in which:
(a) the collected and homogenized animal or human gastric juice is separated in crude parts and supernatant liquid by centrifugalization;
(b) the supernatant liquid is dialysed and lyophilized to obtain a first dry residue;
(c) the first dry residue is purified on a chromatographic column filled with weakly basic modified ion-exchange gel or resin to obtain a first concentrate;
(d) the first concentrate is again dialysed and lyophilized to obtain a second dry residue;
(e) the second dry residue is purified with get chromatography to obtain a second concentrate and
(f) the substance BPC is finally obtained from the second concentrate using dialysis and lyophilisation.

4 more elements

Dependencies are inferred from the claim text.